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Bryan H. Bellaire , Ph.D. Assistant Professor Dept. of Veterinary Microbiology and Preventative Medicine |
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| Research Interests / Areas of Expertise: Our research is focused on determining the host pathogen interactions that take place during bacterial infections. Specifically, the laboratory works to understand how different Brucella species, bacterial pathogens of swine, cattle and humans, causes chronic infections in various mammalian species. Current projects are 1) identifying the underlying mechanism of cytokine induced intracellular resistance to Brucella, 2) delineating the pathogenic factors responsible for long term persistence of the bacteria within individual cells and host tissues, and 3) determining means to disrupt Brucella’s intracellular niche within infected cells. Experimental methods used in this effort are in vitro/in vivo modeling of chronic disease, isogenic mutant construction, recombinant genetics, expression reporter systems, siRNA-knockdown studies, immunofluorescent and confocal microscopy. Education & Certifications: 1995 B.S. Northern Arizona University 2001 Ph.D. Louisiana State University Health Sciences Center-Shreveport Selected Publications: Roux, C.M., N.J. Booth, B.H. Bellaire, J.M. Gee, R.M. Roop II, M.E. Kovach, R.M. Tsolis, P.H. Elzer and D.G. Ennis. 2006. J. Bacteriol. 188(14): 5187-5195. B.C. Reed, C. Cefalu, B.H. Bellaire, J.A. Cardelli, T. Louis, J. Salamon, M.A. Bloecher and R.C. Bunn. 2005. GLUT1CBP(TIP2/GIPC1) Interactions with GLUT1 and Myosin VI: Evidence Supporting an Adapter Function for GLUT1CBP Mol. Bol. Cell. 16(9): 4183-4201. Bellaire, B.H., R.M. Roop II, J.A. Cardelli. 2005. Opsonized virulent Brucella abortus replicate within non-acidic, endoplasmic reticulum negative, LAMP 1 positive phagosomes in human monocytes. Infect. Immun. 73(6): 3702-3713. Valderas, M.W., R.B. Alcantara, J.B. Baumgartner, B.H. Bellaire, G.T. Robertson, W.L. Ng, J.M. Richardson, M.E. Winkler, and R.M. Roop II. 2005. Role of HdeA in acid resistance and virulence in Brucella abortus 2308. Vet. Micro. 20;107(3-4): 307-12. Roop II, R.M., B.H. Bellaire, M.W. Valderas, and J.A. Cardelli. 2004. Adaptation of the brucellae to their intracellular niche. Mol. Micro. 52(3): 621-630. RoopII, R.M., B.H. Bellaire, E. Anderson and J.T. Paulley. 2004. Iron metabolism in Brucella, p. 243-262. In I. Lopez-Goni and I. Moriyon (ed.), Brucella: Molecular and cellular biology. Horizon Scientific Press. Bellaire, B.H., P.H. Elzer, C.L. Baldwin, and R.M. Roop II. 2003. Production of the siderophore 2,3-dihydroxybenzoic acid is required for wild-type growth of Brucella abortus in the presence of erythritol under low iron conditions in vitro. Infect Immun. 71(5): 2927-832.Bellaire, B.H., P.H. Elzer, S.D. Hagius, J.V. Walker, N.J. Booth, M.D. Edmonds, C.L. Baldwin, R. Freeland and R.M. Roop II. 2003. Genetic organization and iron-Responsive regulation of the Brucella abortus 2,3-dihydroxybenzoic acid biosynthesis operon, a cluster of genes required for wild-type virulence in pregnant cattle. Infect Immun. 71(4): 1794-1803.Parent, M.A., Bellaire, B.H., Murphy, E.A., R.M. Roop II, P.H. Elzer and C.L. Baldwin. 2002. Brucella abortus siderophore2,3-dihydroxybenzoic acid (DHBA) facilitates intracellular survival of the bacteria. Microb. Pathog. 32(5): 239-248. Bellaire, B.H., C.L. Baldwin, P.H. Elzer and R.M. Roop II. 1999. The siderophore 2,3-dihydroxybenzoic acid is not required for virulence of Brucella abortus in BALB/c mice. Infect. Immun. 67: 2615-2618.
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