Research Interests / Areas of Expertise:
Dr. Miller’s research focuses on the interface between viruses and the cells they infect, with a strong emphasis on the ways in which segmented double-stranded RNA viruses of the Reoviridae family commandeer cellular space and proteins to replicate their genomes, translate their proteins, and assemble into progeny virions.  The primary projects within the Miller laboratory focus on understanding the inner workings of distinct inclusion structures (termed viral factories) formed within the cellular cytoplasm during viral infection, and determining what drives the creation of these structures, and what role they play in regulation of viral RNA transcription, translation and replication during infection.  Dr. Miller has also been a key player in the development of a novel protein-protein interaction technology utilizing the capacity of a single reovirus protein to form viral factory-like structures and recruit other proteins to these structures.  A second project in the Miller laboratory is focused on utilizing this technology to identify and define new viral protein-protein interactions, and further, to screen for drugs or small molecules that can inhibit these interactions to identify potential anti-viral therapies.    

Education & Certifications:
B.A.  University of Missouri-Columbia
Ph.D. University of Missouri-Columbia Medical School
Post-doctoral Fellow  Harvard Medical School

Teaching:
Molecular Virology

Selected Publications:
T. J. Broering, M. M. Arnold, C. L. Miller, J. A. Hurt, P. L. Joyce, and M.L. Nibert.  2005.  Carboxyl-proximal regions of mammalian reovirus non-structural protein mNS necessary and sufficient for forming factory-like inclusions.  Journal of Virology 79(10):6194-6206.

C. L. Miller, J.S.L. Parker, J. B. Dinoso, C. Piggot, , M. J. Perron and M.L. Nibert. 2004. Increased ubiquitination and other covariant phenotypes attributed to a strain- and temperature-dependent defect of reovirus core protein  m2.  Journal of Virology 78(19): 10291-10302.

A. Helander, C. L. Miller, K.S. Myers, M. R. Neutra, and M. L. Nibert. 2004. Protective immunoglobulin A (IgA) and IgG antibodies bind to overlapping intersubunit epitopes in the head domain of type 1 reovirus adhesin s1.  Journal of Virology 78(19): 10695-10705.

T.J. Broering, J. Kim, C. L. Miller, C. Piggott, J. Dinoso, M. Nibert, and J.S.L. Parker.  2004.  Reovirus non-structural protein mNS recruits viral core surface proteins and entering core particles to factory-like inclusions. Journal of Virology.  78(4):  1882-1892. 

C. L. Miller T.J. Broering, J.S.L. Parker, M.M. Arnold and M.L. Nibert.  2003. Reovirus sNS protein localizes to inclusions through an association requiring the N-terminus of mNS.  Journal of Virology  77(8):  4566-4576 (Cover Illustration).

C. L. Miller and D. Pintel.  2002.  Interaction between Parvovirus NS2 protein and nuclear export factor Crm1 is important for viral egress from the nucleus of murine Cells.  Journal of Virology  76(7):  3257-3266.

C. L. Miller and D. Pintel.  2001.  The NS2 protein generated by the Parvovirus minute virus of mice (MVM) is degraded by the proteasome in a manner independent of ubiquitin chain elongation or activation.  Virology 285:  346-355.

A. Eisenstark, Yallaly, P., Ivanova, A., and C. Miller.  1995.  Genetic mechanisms involved in cellular recovery from oxidative stress.  Arch Insect Biochem Physiol  29(2):  159-173.

A. Eisenstark,  Miller, C., Jones, J., and S. Leven.  1995.  Escherichia coli genes involved in cell survival during dormancy:  role of oxidative stress.  Biochem Biophys Res Commun  188(3): 1054-9.

A. Ivanova,  Miller, C., Glinsky, G., and A. Eisenstark.  1994. Role of rpoS (katF) in oxyR-independent regulation of HP1 in Escherichia coli.  Mol Microbiol 12(4):  571-578.


Honors & Awards:

K22, NIH, NIAID Career Development Award, 2005

Postdoctoral Fellow, NIH, NIAID, 2003-2005
Ruth Kirchstein Individual NRSA Training Grant                                         

Postdoctoral Fellow, NIH, NIAID, 2002-2003
Institutional NRSA Training Grant
Infectious Disease and Basic Microbiological Mechanisms
Harvard Medical School

Ernst Postdoctoral Fellow, 2001-2002
Harvard Medical School

Pre-doctoral Fellow, NIH, NIAID, 1995-1999
Institutional NRSA Training Grant                            
Department of Molecular Microbiology and Immunology
University of Missouri-Columbia School of Medicine

Donald K. Anderson Teaching Award, 1995-1996
University of Missouri-Columbia


Professional Society Memberships:
American Society of Virology, Associate Member, 2001-2005

American Association for the Advancement of Science, 2005