Rabbits

Rabbits are commonly used to produce polyclonal anti-PCV2-antibodies for usage in immunohistochemistry assays (Sorden et al., 1999). Quintana et al. (2002) inoculated 16 female New Zealand rabbits intranasally with each PCV2 and PCV1. Necropsies were performed at 3, 7, 10, 14, and 20 DPI. None of the rabbits seroconverted to PCV1 or PCV2, PCV2 nucleic acids as determined by ISH were not detected in any of the rabbits, and there were no PCV2-associated microscopic lesions (Quintana et al., 2002). Fifteen rabbits were found to be negative for PCV2-specific antibodies by IPMA (Rodríguez-Arrioja et al., 2003) and 60 rabbits were negative for PCV1-specific antibodies by IFA (Allan et al. 1994).

References:

Allan GM, Phenix KV, Todd D, McNulty MS: Some biological and physico-chemical properties of porcine circovirus. Zentralbl Veterinarmed B. 41:17-26, 1994

Quintana J, Balasch M, Segalés J, Calsamiglia M, Rodríguez-Arrioja GM, Plana-Durán J, Domingo M: Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice. Vet Res. 33:229-237, 2002

Rodríguez-Arrioja GM, Segalés J, Domingo M, Plana-Duran J: Lack of PCV-2 infection in non-porcine species in Spain. Vet Rec. 153:371-37, 2003

Sorden SD, Harms PA, Nawagitgul P, Cavanaugh D, Paul PS: Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. J Vet Diagn Invest. 11:528-530, 1999